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KMID : 0923620180180040026
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Immune Network
2018 Volume.18 No. 4 p.26 ~ p.26
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Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations
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Kim Ki-Hyang
Kim Sang-Hyun
Jung Ho-Hyun
Moon Jun-Hyeok
Jeong Seong-Un
Yu Kyeong-Ae
Lee Chong-Kil
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Abstract
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Thapsigargin (TGN) is a potent and selective inhibitor of sarco-endoplasmic Ca2+-ATPase, leading to rapid elevation of cytoplasmic Ca2+ concentration. Previous reports have shown that TGN increases the production of various cytokines from macrophages and dendritic cells. Here, we examine the effects of TGN on murine T cells. Nanomolar concentrations of TGN are a significant inducer of IL-2 production with full activity at 50 nM. Micromolar concentrations of TGN, however, are inhibitory to IL-2 production and T cell proliferation. The IL-2 production-inducing activity of TGN is much more prominent when T cells are primed with concanavalin A or anti-CD3 mAb, and is due to the increase of cytoplasmic Ca2+ concentration. TGN at 50 nM does not affect interferon-gamma or IL-4 production from T cells. Thus, the present study shows that low nanomolar concentrations of TGN could be useful in potentiating IL-2 production from antigen-primed T cells.
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KEYWORD
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Thapsigargin, Cytoplasmic Ca2+, T cells, IL-2, Phorbol myristate acetate
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